Formulation and Activity Test of Itchy Leaf Extract Anti-Acne Gel (Laportea aestuans) Against Propionibacterium acne Bacteria

Risna Risna; Gabriela Welma Litaay

doi:10.55927/fjst.v4i11.291

Authors

Risna Risna Poltekkes Kemenkes Jayapura
Gabriela Welma Litaay Universitas Papua

DOI:

https://doi.org/10.55927/fjst.v4i11.291

Keywords:

Itchy Leaf, Gel, Antibacterial, Propionibacterium Acnes

Abstract

Laportea aestuans (commonly known as “daun gatal”) contains various secondary metabolites with potential antibacterial activity. This study aimed to formulate an ethanol extract of Laportea aestuans leaves into a gel dosage form and to evaluate its antibacterial effectiveness against Propionibacterium acnes, the primary bacterium responsible for acne. An experimental design was employed by preparing gel formulations containing extract concentrations of 5%, 10%, and 15%. The antibacterial activity was evaluated using the diffusion method, while the physical quality of the gel was evaluated through organoleptic observations, homogeneity, pH, spreadability, and adhesion tests. The results showed that all gel formulations demonstrated good physical quality. The antibacterial assay showed inhibition zone diameters of 13.7 mm, 14.8 mm, and 16.7 mm for the 5%, 10%, and 15% formulations, respectively, which were classified as strong inhibition. Therefore, it can be concluded that the anti-acne gel containing ethanol extract of Laportea aestuans leaves effectively inhibits the growth of Propionibacterium acnes.

References

Febrianto, Y., & Alvyani, J. M. (2020). Formulasi Dan Evaluasi Sediaan Gel Ekstrak Etanol Daun Cabai Rawit (Capsicum frutescens L.) Dengan Variasi Carbopol Dan Cmc Na Sebagai Gelling Agent. SCIENTIA J. Far. Kes, 10(2), 136–145.

Hakim, A. R., & Saputri, R. (2020). Narrative Review : Optimasi Etanol Sebagai Pelarut Senyawa Flavonoid dan Fenolik. Jurnal Surya Medika (JSM), 6(1), 177–180.

Hanip, A. I., Mayasari, D., & Indriyanti, N. (2021). Formulasi dan Uji Aktivitas Gel Anti Jerawat Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi Linn). Proceeding of Mulawarman Pharmaceuticals Conferences, 14, 1–7.

Hikmah, F. N., Malahayati, S., & Fitri Nugraha, D. (2023). Formulasi Dan Evaluasi Sediaan Serum Gel Ekstrak Bunga Melati (Jasminum Sambac L.) Journal of Pharmaceutical Care and Sciences (Vol. 3, Issue 2).

Kambu, J., Kaniawati, M., & Idar, I. (2024). Evaluasi Aktivitas Antibakteri Ekstrak Etanol Daun Gatal (Laportea interrupta dan Laportea aestuans) terhadap Staphylococcus aureus dan Escherichia coli Menggunakan Metode Difusi Cakram dan Bioautografi . Kimia Padjajaran. 3(1), 40–53.

Kusmana, C., & Hikmat, A. (2015). The Biodiversity of Flora in Indonesia. Journal of Natural Resources and Environmental Management, 5(2), 187–198.

Madelina, W., & Sulistiyaningsih. (2018). Review: Resistensi Antibiotik Pada Terapi Pengobatan Jerawat. FARMAKA. 16 (2).

Marina, M., Zahran, I., & Ervianingsih, E. (2024). Formulasi dan Uji Efektivitas Acne Spot Gel Kstrak Daun Ketepeng Cina (Cassia Alata L.) Terhadap Bakteri Propionibacterium Acnes. Jurnal Mandala Pharmacon Indonesia, 10(2), 527–536.

Niken, N., Yusuf, R. N., & Annita, A. (2022). Uji Aktivitas Antibakteri Ekstrak Daun Jambu Biji (Psidium guajava L.) terhadap Pertumbuhan Bakteri Escherichia coli. Bioscientist : Jurnal Ilmiah Biologi, 10(2), 726.

Nofriyanti, Sari, S. P., Iskandar, B., Firmansyah, F., Ikhtiaruddin, I., & Susanti, E. (2021). Formulasi dan Uji Aktivitas Antibakteri Gel Ekstrak Kering Rimpang Kunyit (Curcuma domestica Cal.) Terhadap Propionibacterium acnes. Majalah Farmasi Dan Farmakologi, 25(3), 84–87.

Nurrosyidah, I. H., Hermawati, R., & Asri, M. (2019). Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Etanol Pegagan (Centela Asiatica L.) Terhadap Bakteri Staphylococcus Aureus Secara In Vitro. In J-PhAM Journal of Pharmaceutical Care Anwar Medika (Vol. 45, Issue 2).

Pertiwi, K. K., & Fernanda, D. S. (2019). Aktivitas Antibakteri Herba Daun Gatal (Laportea interupta L. Chew) terhadap Staphylococcus aureus dan Escerichia coli. J-HESTECH (Journal Of Health Educational Science And Technology), 2(1), 43.

Piramitha, A., Soesanto, A., & Riyanto, P. (2017). Pengaruh Pemberian Suplementasi Likopen Terhadap Derajat Keparahan Akne Vulgaris. Jurnal Kedokteran Ponorogo (Vol. 6, Issue 2).

Rahmah, A. F., Mursyid, M., Hurria, H., Meylany, F., Husna, A., & Nabilah, A. (2024). Formulasi Sediaan Mouthwash Antibakteri Infusa Daun Mengkudu (Morinda citrifolia L.) dan Daun Serai (Cymbopogon citrates) Terhadap Streptococcus mutans. Jurnal Mandala Pharmacon Indonesia, 10(2), 432–438.

Ramadhani, M. A., Nadifah, S. D., Putri, N. A., & Sulastri. (2024). Uji Aktivitas Antibakteri Berbagai Ekstrak Tanaman Herbal Terhadap Staphylococcus epidermidis Antibactery Activity Test Of Various Plants Herbs Againts Staphylococcus epidermidis. Journal of Research in Pharmacy, 4(1), 65–76.

Risna, R. (2023). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Matoa (Pometia Pinnata J. R & G.Forst.) terhadap Pertumbuhan Staphylococcus Aureus dan Escherichia Coli. Jurnal Keperawatan Silampari, 6(2), 1139–1149.

Sawiji, R. T., & Sukmadiani, N. W. A. (2021). Formulasi Sediaan Salep Ekstrak Daun Puring (Codiaeum variegatum L.) Dengan Basis Hidrokarbon Dan Larut Air. Indonesian Journal of Pharmacy and Natural Product. Vol.4 (2)

Setiawan, A. (2022). Keanekaragaman Hayati Indonesia: Masalah dan Upaya Konservasinya. Indonesian Journal of Conversation, 11(1), 13–21.

Setyawan, R., Dwi, C., Masrijal, P., Hermansyah, O., Rahmawati, S., Intan, R., Sari, P., & Cahyani, A. N. (2023). Formulasi, Evaluasi Dan Uji Stabilitas Fisik Sediaan Gel Antioksidan Ekstrak Tali Putri (Cassytha filiformis L). In Bencoolen Journal of Pharmacy 2023 (Vol. 3, Issue 1).

Simaremare, E. S., Uopmbin, E., & Gunawan, E. (2019). Studi Etnobotani Daun Gatal Oleh Masyarakat Kiwirok Papua Etnobotani Study Of Itchy Leaves By Communities Kiwirok Papua. In Pharmaceutical Journal of Indonesia (Vol. 16).

Subaryanti, Sabat Dwi Meianti, D., & Trijuliamos Manalu, R. (2022). Potensi Antimikroba Ekstrak Etanol Daun Gatal (Urticastrum decumanum (Roxb.) Kuntze) Terhadap Pertumbuhan Staphylococcus aureus dan Candida albican. Jurnal Ilmu Kefarmasian. 15(2).

Tjitrarukmana, S. (2022). Formulation and Evaluation of Lime Peel Extract (Citrus aurantiifolia) Gel with Semi-Refined Carrageenan and Glucomannan as Gelling Agent. FITOFARMAKA: Jurnal Ilmiah Farmasi, 12(2), 135–143.

Yasacaxena, L. N., Defi, M. N., Kandari, V. P., Weru, P. T. R., Papilaya, F. E., Oktafera, M., & Setyaningsih, D. (2023). Review: Extraction of Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) and Activity As Antibacterial. Jurnal Jamu Indonesia, 8(1), 10–17.