The Role of Ultrasonic Generators as Killers Gram + and Gram – Bacteria

Abstract

It has created an ultrasonic generator as a destroyer of E.Coli bacteria.The range of frequency that produced by this ultrasonic generator is around 40 kHz up to 50 kHz. From the observation of death of E.Coli bacteria which is impacted by the ultrasonic wave radiation to the frequency variant used at the closed rank of 10-8 cfu/ml ( coloni forming unit / ml ) has obtained that the percentage of E.Coli bacteria death everage is increasing as well as the increasing frequency had been used. At the frequency of 40 kHz able to destroy E.Coli bacteria at the amount of 9,3 %, at the frequency of 42 kHz able to destroy at the amount of 14,1 %, at the frequency of 45 kHz able to destroy at the amount of 26,1 %, at the frequency of 48 kHz able to destroy at the amount 29,7 % and at the frequency of 50 kHz able to destroy at the amount of 38,8 %.By checking the result ot colouring gram of bacteria, we found that the cell of E.Coli bacteria has changed after we doing a radiation to that bacteria by using ultrasonic wave , such as ; the shape of bacteria is getting smaller, partition of the cell is cracked and lysis is occured on it.